Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.
نویسندگان
چکیده
منابع مشابه
More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis
Objective. To characterise fragmentation patterns and amino acid composition of MUC2 and MUC5AC in chronic sinusitis. Methods. Antigenic identity of purified sinus mucins was determined by ELISA. Fragmentation patterns of a MUC5AC rich sample mucin were analysed by Sepharose CL-2B gel chromatography. Samples, divided into one MUC2 rich and one MUC5AC rich group, were subjected to sodium dodecyl...
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Gastric mucins are high molecular weight extracellular glycoproteins that play a major role in the protection of the gastrointestinal tract and besides that they are also involved in many disease processes. In the present study, size exclusion chromatography under non-degrading conditions was used to study the fragmentation pattern of native gastric mucins. The samples of gastric mucins of diff...
متن کاملA Simpler and Faster Version of Two-Dimensional Gel Electrophoresis Using Vertical, Mini SDS-PAGE Apparatus
We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...
متن کاملBiosynthesis of mucins (MUC2-6) along the longitudinal axis of the human gastrointestinal tract.
Little is known about the biosynthesis of mucin molecules in humans. Our aim was to examine the mucin biosynthesis (MUC2-6) along the longitudinal axis of the healthy human gastrointestinal tract. Biopsies of human stomach and small and large intestine were metabolically labeled with 35S-labeled amino acids, [35S]sulfate, or[3H]galactose, immunoprecipitated with antibodies against MUC2-6, and a...
متن کاملMUC2 is the prominent colonic mucin expressed in ulcerative colitis.
BACKGROUND It has been shown that MUC2 is the prominent mucin synthesised in healthy colon. AIM To identify the predominant mucins in ulcerative colitis (UC) and to study their biosynthesis. METHODS AND RESULTS Mucin was purified from UC resection specimens. This mucin on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) presented as one, high molecular weight, periodic a...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 310 ( Pt 3) شماره
صفحات -
تاریخ انتشار 1995